ESTs

If you would like to request Platynereis dumerilii EST clones please fill in this form.

If you are interested in more than ~20 clones, you may want to contact us for a potential collaboration.

You can identify potential clones for your genes of interest on our BLAST page.

Details of the library

Platynereis dumerilii full-length normalized cDNA library

Vector:

pCMV-SPORT6.1

Cells:

DH10BTM T1 (T1 and T5 phage resistance)

Average Insert Size:

1.8 kb (with NotI and EcoRI)

Number of primary clones:

1.9 x 1011 cfu

Number of clones sequenced:

~90.000

First strand primer:

Oligo dT

Reverse transcriptase:

SuperScriptTM III

Primer(s) for Sequencing:

T7 (antisense) and SP6 (sense)
or
M13 Forward GTA AAA CGA CGG CCA GT
M13 Reverse CAG GAA ACA GCT ATG AC

This work has been supported by a Sequencing Grant M.IF.A.ENTW8050 from the Max Planck Society.